yvdA [2019-08-09 13:43:47]

similar to carbonic anhydrase

locus: BSU_34670

pI: 6.88

mw: 21.57 kDa

protein length: 197 aa

gene length: 594 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 3,561,590 → 3,562,183

The protein

Protein family

• beta-class carbonic anhydrase family (with [protein|5D96DF2F32050BBCEC3811843398FE84DE88F6DF|YbcF] and [protein|F942C0F4618FACEF35ACA629355BAC288556E7B6|YtiB], according to UniProt)

Paralogous protein(s)

• [protein|F942C0F4618FACEF35ACA629355BAC288556E7B6|YtiB]
• [protein|5D96DF2F32050BBCEC3811843398FE84DE88F6DF|YbcF]: (29.4%)

Structure

• [PDB|1G5C] (from Methanobacterium thermoautotrophicum 40% identity) [pubmed|11096105]

Expression and Regulation

Operons

• genes: [gene|E00889289B07BE78CC0C8E0962A55C3B14871E00|yvdA]-[gene|7028580E25DD6C1D49A14F29DDCC02012F94FED7|yvdB]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B632 (yvdA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1631 NBRP B. subtilis, Japan]
• BKE34670 (Δ[gene|E00889289B07BE78CC0C8E0962A55C3B14871E00|yvdA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCCCATCCATATGATT, downstream forward: _UP4_TCATAAGGGAGGTTATAACA
• BKK34670 (Δ[gene|E00889289B07BE78CC0C8E0962A55C3B14871E00|yvdA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCCCATCCATATGATT, downstream forward: _UP4_TCATAAGGGAGGTTATAACA

References

• 22383849,11096105